Abstract
Benzoyl hydrazine derivatives of reducing carbohydrates were analyzed by high performance capillary electrophoresis with on-tube ultraviolet detection. The derivatives of 9 reducing monosaccharides were completely separated in ca. 30 min. using a capillary tube (50 μm i.d., 62 cm) and 200 mM borate buffer, pH 10.8, as carrier. On-column UV detection at 220 nm was able to achieve sensitive detection down to 30‐40 femtomole levels. The experimental parameters for optimum precolumn are discussed along with derivatization and separation. It was demonstrated that this method allows quantification of reducing carbohydrates with high accuracy and reproducibility.
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More From: Journal of Liquid Chromatography & Related Technologies
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