Abstract
Penetrant testing (PT) is a Nondestructive testing (NDT) method used for identifying and revealing surface cracks and defects in components. Using bacteria-based suspension as a fluorescent penetrant leads a new approach to PT with the advantages of using eco-friendly chemicals, fewer steps for test process and achieving low cost. The aim of the current study is to synthesize a new fluorescein isothiocyanate (FITC) conjugated Escherichia coli (E. coli) bacteria (FITC-E. coli) as fluorescent penetrant for detection of cracks on test materials. For this purpose, we tested performance of proposed bacteria suspension by means of its detection sensitivity using standardize test panels. Crack centers (6.35, 3.97 and 2.38 mm) and defects (0.2 × 0.4, 0.3 × 0.4 and 0.4 × 0.4 mm) were successfully detected via FITC-E. coli. Experimental results showed that the FITC-E. coli is efficient for detecting the cracks and defects. Research into development of bacteria-based fluorescent penetrant may bring new opportunities for NDT applications in the future.
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