Use of Antimicrobial Peptides Secreted by Trichoderma Micromycetes to Stimulate Embryogenic Cultures of Larix sibirica

Abstract

In vitro cultivation of embryogenic cultures of Siberian larch on AI medium supplemented with different concentrations of antimicrobial peptides of two Trichoderma species, T. citrinoviride (strain TYVI 4/11) and T. viride (strain 346), has been performed to achieve a direct antimicrobial effect and initiate the mechanisms of induced resistance (regulatory function of embryogenic cultures) as well as to study the morphogenesis and growth activity of regenerant plants. The experiment was arranged using four cell lines, CL4, CL5, CL6, and CL12, differing in their embryogenic activity. The effect of treatment of cell cultures with Trichoderma peptides was manifested via significant growth stimulation of proliferating embryogenic cell lines, root growth stimulation, and formation of callus excrescences on regenerant plants. No difference between the treated and untreated variants was observed in seedlings. Conceivably, such peptide treatment may provide an increased immunity of soil-grown seedlings in forest nurseries. Study of the effect of biocontrol strains’ use in a plantation reforestation to stimulate the growth and development of calluses and embryos and to obtain disease-resistant regenerant plants of conifer species will provide the development of a clonal silviculture, a new trend that recently appeared abroad (Park 2002, 2014).