Use of anaesthetics with the silver-lip pearl oyster, Pinctada maxima (Jameson)

Abstract

Mortality, lower pearl retention and low pearl quality can be major problems in cultured pearl production. Anaesthetics may have a role in the pearl seeding process and this aspect was investigated in this study. Seven anaesthetic treatments, 3 mL L−1 2-phenoxyethanol, 500 mg L−1 and 1200 mg L−1 of benzocaine, 1.5 mL L−1 clove oil, 0.25 mL L−1 and 0.4 mL L−1 menthol liquid, and 2.5 mL L−1 propylene phenoxetol were assessed for their ability to relax the silver-lip pearl oyster, Pinctada maxima. Of the 27 oysters exposed to each treatment, the highest proportion of relaxed oysters (96.3, 88.9 and 88.9%) and the shortest exposure times required for anaesthesia (13.8±6.4, 10.5±7.9 and 15±7.1 min), were recorded for the treatments of 3 mL L−1 2-phenoxyethanol, 1200 mg L−1 of benzocaine, and 2.5 mL L−1 propylene phenoxetol, respectively. In contrast, none of the oysters exposed into 0.25 mL L−1 menthol liquid became relaxed and most oysters exposed into 1.5 mL L−1 clove oil died during the experiment. Oysters exposed to 3 mL L−1 2-phenoxyethanol and 1200 mg L−1 benzocaine remained relaxed for up to 30 min while the number of relaxed oysters exposed to 2.5 mL L−1 propylene phenoxetol decreased during that time. With the exception of oysters exposed to clove oil, all relaxed oysters recovered within 2 h of being placed back into seawater and there was close to 100% survival after one month.