Abstract
Caulerpa taxifolia is a high profile introduced alga in the Mediterranean Sea and California. It is native to tropical and subtropical regions of Australia. However, during 2000/01, 7 locations with abundant C. taxifolia were discov- ered in temperate waters around Sydney. To overcome limita- tions of resolution in previous studies using ITS (internal tran- scribed spacer) rDNA sequencing, we developed amplified fragment length polymorphism (AFLP) markers for C. taxi- folia, using 4 primer combinations. With this technique we found a close relationship between isolates from Moreton Bay (native) and Lake Conjola (introduced). The Mediterranean samples, which previously could not be separated from the other isolates, formed a discrete cluster supported by high bootstrap values. AFLP had greatly improved resolution over other molecular markers, making it a promising technique for further studies on the population structure of C. taxifolia and the assignment of source populations.
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