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Abstract
AccuProbe tests for mycobacteria (Gen-Probe) cannot be performed directly on Bactec 13A cultures because of interfering substances. This problem can be circumvented by subculturing to Bactec 12B media. Artifactual chemiluminescence greater than the positive cutoff was seen in 15 of 19 13A cultures containing Mycobacterium avium complex compared with 0 of 19 in 12B subcultures. Of the subcultures, 89% were tested after overnight incubation.
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