Abstract
Acetone as an aphid anesthetic was compared with mechanical removal of aphids from leaves to enhance the harvesting and handling of large populations of the aphid Myxus persicae. The effect of these two procedures on aphid virus transmission efficiency was assessed with a persistent and a nonpersistent virus using potato leafroll virus (PLRV) and papaya ringspot virus type W (PRSV-W), respectively. The use of acetone was 8-10 times faster and about 30% more efficient than the camel's-hair brush technique for harvesting M, persicae from leaves of turnip, Malva parviflora, and sweet pepper. Even small nymphs could be collected and handled easily, indicating an additional benefit of the acetone method
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