Use of a synthetic form Avrodes for transfer of leaf rust resistance from Aegilops speltoides to common wheat

R O Davoyan,E A Salina,I V Bebyakina,I G Adonina,D S Mikov,E D Badaeva,A N Zinchenco,E R Davoyan,Y S Zubanova

doi:10.18699/vj17.284

Abstract

Diploid wild relative of wheat – Aegilops speltoides – is a valuable source of genes for resistance to diseases. The synthetic form Avrodes (BBAASS) was used as a bridge to transfer leaf rust resistance genes from Ae. speltoides to common wheat. Introgression lines obtained from crosses of Avrodes and susceptible common wheat cultivars were evaluated in a field leaf rust nursery. Resistance levels varied from high to moderate. Testing of lines with the use of molecular markers has shown that some lines have the Lr28 and Lr35 genes inherited from synthetic form Avrodes. The majority of resistance lines have not been found to carry these genes. The Lr47 and Lr51 genes were not identified in the Avrodes and introgression lines. The analysis of chromosome pairing in F1 hybrids showed that the transfer of a genetic material from Avrodes to common wheat basically occurs through translocations. Lines with translocations on chromosomes 2D and 5D were identified by C-banding and FISH. The translocations differed in chromosomal location from known leaf resistance genes transferred to common wheat from Ae. speltoides. Hence it was assumed that new genes were introduced into the common wheat genome from Ae. speltoides. Introgression lines have been studied for productivity and technological qualities of grain. Lines AA60n9 and D37n10 combine high resistance to leaf rust with good characteristics of productivity and technological qualities of grain. The received results demonstrate a genetic diversity and a value of the investigated introgression lines for breeding of common wheat.

Highlights

Ключевые слова: Triticum aestivum; Aegilops speltoides; интрогрессивные линии; устойчивость к листовой ржавчине; цитологический анализ; молекулярные маркеры; продуктивность и технологические качества зерна
Introgression lines obtained from crosses of Avrodes and susceptible common wheat cultivars were evaluated in a field leaf rust nursery
Testing of lines with the use of molecular markers has shown that some lines have the Lr28 and Lr35 genes inherited from synthetic form Avrodes

Summary

Генетика и селекция растений

Полученные результаты свидетельствуют о генетическом разно образии и ценности исследуемых интрогрессивных линий для селекции мягкой пшеницы на устойчивость к листовой ржавчине. Как цитировать эту статью: Давоян Р.О., Бебякина И.В., Давоян Э.Р., Миков Д.С., Бадаева Е.Д., Адонина И.Г., Салина Е.А., Зинченко А.С., Зубанова Ю.С. Использование синтетической формы Авродес для передачи устойчивости к листовой ржавчине от Aegilops speltoides мягкой пшенице. В представленной работе приведены результаты оценки интрогрессивных линий мягкой пшеницы Triticum aestivum/Авродес по устойчивости к листовой ржавчине, наличию у них транслокаций и известных генов устойчивости от Ae. speltoides, по продуктивности и технологическим характеристикам зерна. Материалы и методы Исходным материалом служили 115 интрогрессивных линий мягкой пшеницы (BC4F6 – BC2F22), полученные от скрещивания синтетической формы Авродес с восприимчивыми к листовой ржавчине сортами селекции Краснодарского НИИ сельского хозяйства им. Статистический анализ полученных результатов проводили методом дисперсионного анализа с использованием программы AGROS-2.10

Nucleotide sequence

To t a l

Number of lines total with translocations with substituted chromosomes

Overall score