Abstract
A simple procedure for the isolation and determination of p-dichlorodiphenylacetic acid (DDA) in urine and other biological fluids has been presented. The method, utilizing inexpensive laboratory apparatus, commercially available Florisil, and prepared thin-layer chromatography sheets, permits the identification and estimation of DDA in the order of 0.005 ppm. The average recovery of from 5 to 50 μg DDA added to DDA-free urine was 68.4%. Urinary DDA excretion of 13 human subjects picked at random from the general population was found to average 46 μg/day, ranging from 13 to 117 μg/day.
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