Abstract
Salmonella enterica subsp. enterica ( S .) serovar Enteritidis is one of the main pathogens involved in food-borne diseases worldwide. In epidemiological investigations of food-related salmonellosis, subtyping is necessary to improve preventive and control measures. Single-enzyme amplified fragment length polymorphism (SE-AFLP) analysis is a modified AFLP that uses only one restriction enzyme to produce DNA fragments that are selectively amplified by PCR. In order to assess the applicability of SE-AFLP in S. Enteritidis typing, one hundred and eight strains isolated from poultry, swine and also from human salmonellosis outbreaks in Southern Brazil were analyzed. Strains from other countries and six different S. enterica serovars were also included as controls. SE-AFLP was able to distinguish S . Enteritidis from the other S. enterica serovars analyzed. However, most of S . Enteritidis strains isolated from poultry, salmonellosis outbreaks and most of the strains from other countries shared the same predominant pattern. The low genetic diversity identified in S . Enteritidis suggests that the strains analyzed are clonally related and one predominant SE-AFLP genotype is widely spread in Southern Brazil.
Highlights
Salmonella enterica subsp. enterica (S.) serovar Enteritidis has emerged worldwide as the most common bacteria isolated from human salmonellosis [1], and infections have occurred in Brazil since 1990s [2]
Single-enzyme amplified fragment length polymorphism (SE-Amplified-fragment length polymorphism (AFLP)) analysis evaluated DNA segments obtained from HindIII digestion, which were distributed all over the Salmonella genome
2.0, 2.5 and 3.0 mM were initially tested with each prienterica subsp. enterica serovar Enteritidis isolates
Summary
Salmonella enterica subsp. enterica (S.) serovar Enteritidis has emerged worldwide as the most common bacteria isolated from human salmonellosis [1], and infections have occurred in Brazil since 1990s [2].Since S. Enteritidis has a wide range of animal reservoirs and a high spread potential, improvements in epidemiological surveillance are currently necessary. In this sense, accurate characterization is crucial to differentiate pathogenic strains from less-pathogenic ones, as well as to study strain origin, evolution and major infection routes, which will direct preventive and control measures. Because of the predominance of some phage types [3,4,5], molecular typing became important for strain characterization and has been associated with the traditional phenotypic methods to improve discriminatory power.
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