Abstract
The Doehlert design was used in optimizing the analysis of selected tropane alkaloids (including hyoscyamine, scopolamine and littorine) by micellar electrokinetic capillary chromatography. Three variables, i.e., pH, SDS concentration and organic modifier percentage were investigated. Resolutions as well as analysis time, generated power and current were established as responses. A model was obtained by linear multiple regression of a second-degree mathematical expression. The Doehlert design structure allows to study the response surfaces with a good quality of the parameters estimation of the quadratic model. Thus, it is possible to obtain the region in which the optimum values of such variables are simultaneously achieved. From the models, the most favorable conditions were determined by optimizing the resolution between hyoscyamine and littorine –two positional isomers– and by setting the other responses at threshold values. Successful results were obtained using a 30 m M borate–phosphate buffer at basic pH (8.7) in the presence of 40 m M sodium dodecyl sulfate and 16.5% acetonitrile. Results were compared with a previous study in which a systematic investigation of the operating parameters was carried out.
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