Abstract
Relationships betweenRF values and mobile-phase composition have been determined for moderately polar pesticides in normal-phase systems (NP) of the type silica-non-polar diluent (heptane)-polar modifier (ethyl acetate, tetrahydrofuran, or dioxane) and in reversed-phase systems (RP) of the type octadecyl silica-water-polar modifier (acetonitrile, methanol, or tetrahydrofuran). These relationships constitute a retention database which has enabled choice of the optimum conditions for preparative column chromatographic separation of pesticides into fractions; these were then applied to a silica plate and chromatographed. The plate was videoscanned, furnishing a real picture of the plate showing complete separation of the pesticide fractions.
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