USE OF A 3’ RNA SEQUENCING APPROACH TO DETERMINE DIFFERENTIAL TRANSCRIPT EXPRESSION AND ALTERNATIVE POLYADENYLATION SITE USAGE BETWEEN AGE MATCHED, HEALTHY AND OSTEOARTHRITIC KNEE CARTILAGE

Abstract

Purpose: Alternative polyadenylation is a mechanism that introduces diversity into transcript structure, generating different length gene transcripts that can ultimately affect protein expression and function. So far, the role of alternative polyadenylation in osteoarthritis has not been extensively examined. This study used QuantSeqRev, a 3’ end RNA-Seq approach, to examine how polyadenylation patterns are affected by the disease.