Abstract
The suitability of 103Ru-labelled tris (1,10-phenanthroline) ruthenium (II) chloride in low concentrations as a marker in digestion studies with sheep has been investigated. The 103Ru complex was strongly adsorbed by dietary ingredients and the particulate fractions of digesta. In studies with five diets, 93–102 per cent of the 103Ru complex administered into the rumen was recovered from the faeces; 0·4 per cent or less was recovered from the urine. Studies with one diet showed that no absorption occurred in the stomach. The overall metabolic activity of the rumen microbiota was not affected by the 103Ru complex in the concentrations used. It was concluded that the 103Ru complex could be a useful marker in a variety of digestion studies. As 103Ru and 51Cr can be assayed simultaneously the 103Ru complex may conveniently be used in digestion studies together with the 51Cr complex of ethylenediamine tetraacetic acid, a water soluble marker not adsorbed by the particulate fractions of digesta.
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