Use development of functional materials in the leaves of Perilla frutescens (L.) Britton var. acuta Kudo (red perilla) with Japanese dietary pickled plum (Umeboshi)

Abstract

Six phenolic compounds and one steroid were isolated from the leaves of Perilla frutescens Britton var. acuta (red perilla) with Japanese dietary pickled plum (Ume). Their structures were determined based on UV, MS, 1H-NMR and 13C-NMR spectroscopic data as β-sitosterol (1), shisonin (2), kaempferol 3-O-rutinoside (3), rosmarinic acid (4), rosmarinic acid methyl ester (5), 4-glucopyranosylcinnamic acid (6), and caffeic acid methyl ester (7). In the bioassay, remarkable superoxide scavenging activity was found in the 1-butanol extract, and 20% methanol-water eluate, 50% methanol-water eluate and methanol eluate of an Amberlite XAD-2. In addition, remarkable inhibitory effect was found in rosmarinic acid and rosmarinic acid methyl ester which are those isolation compounds.