Uropathogenic Escherichia coli in the Urine Samples of Iranian Dogs: Antimicrobial Resistance Pattern and Distribution of Antibiotic Resistance Genes.

Amirhossein Yousefi,Saam Torkan

doi:10.1155/2017/4180490

Abstract

Resistant uropathogenic Escherichia coli is the most common cause of urinary tract infections in dogs. The present research was done to study the prevalence rate and antimicrobial resistance properties of UPEC strains isolated from healthy dogs and those which suffered from UTIs. Four-hundred and fifty urine samples were collected and cultured. E. coli-positive strains were subjected to disk diffusion and PCR methods. Two-hundred out of 450 urine samples (44.4%) were positive for E. coli. Prevalence of E. coli in healthy and infected dogs was 28% and 65%, respectively. Female had the higher prevalence of E. coli (P = 0.039). Marked seasonality was also observed (P = 0.024). UPEC strains had the highest levels of resistance against gentamicin (95%), ampicillin (85%), amikacin (70%), amoxicillin (65%), and sulfamethoxazole-trimethoprim (65%). We found that 21.50% of UPEC strains had simultaneously resistance against more than 10 antibiotics. Aac(3)-IV (77%), CITM (52.5%), tetA (46.5%), and sul1 (40%) were the most commonly detected antibiotic resistance genes. Findings showed considerable levels of antimicrobial resistance among UPEC strains of Iranian dogs. Rapid identification of infected dogs and their treatment based on the results of disk diffusion can control the risk of UPEC strains.

Highlights

Pathogenic urinary tract infections (UTIs) occur in about 14% of dogs throughout their life [1,2,3,4,5]
We found that aac(3)-IV (77%), CITM (52.5%), tetA (46.5%), and sul1 (40%) were the most commonly detected antibiotic resistance genes among the E. coli isolates of Iranian dogs
We found that majority of resistant strains of E. coli had the high prevalence of antibiotic resistance genes and especially aac(3)-IV, CITM, and tetA

Summary

Introduction

Pathogenic urinary tract infections (UTIs) occur in about 14% of dogs throughout their life [1,2,3,4,5]. Uropathogenic Escherichia coli (E. coli) (UPEC) strains are the most significant causative agent of UTIs in both humans and dogs [1,2,3,4,5,6]. It is a Gram-negative, nonsporulating, flagellated, rod-shaped, and facultative anaerobic bacterium which belongs to Enterobacteriaceae family [7,8,9,10,11,12,13]. Total prevalence of UTIs caused by the UPEC strains is about 30–70% [1,2,3,4,5,6]

Methods

Results

Conclusion