Urokinase Regulates Heat Shock Protein 27 to Treat Chronic Glaucoma Rats

Abstract

This study explored urokinase’s effect on the production of heat shock protein 27 in chronic glaucoma rats. 45 SD rats were equally assigned into healthy group, glaucoma group, and urokinase group followed by analysis of intraocular pressure by TONO-PENAVVI tonometer, apoptosis of retinal ganglion cells by TUNEL staining, pathological morphology by H&E staining, levels of SOD, MDA, and NO as well as the expression of HSP27 and p-p38MAPK. After treatment, the intraocular pressure was increased in glaucoma group and decreased in urokinase group (P < 0.05). In addition, glaucoma group showed significantly increased apoptosis rate (P < 0.05) which was decreased in urokinase group (P < 0.05). In glaucoma group, the nerve fibers were disorderly arranged and ganglion cells were greatly reduced which were improved in urokinase group. Compared to healthy group, glaucoma group had lower MDA level and higher SOD levels (P < 0.05) which were reversed in urokinase group (P < 0.05). HSP27 and P-P38MAPK levels in glaucoma group were higher than healthy group (P < 0.05) and urokinase group (P < 0.05). In conclusion, urokinase can reduce the apoptosis of retinal ganglion cells in glaucoma rats and protect the function of the optic nerve by reducing the level of HSP27.