Abstract
Purpose: To determine a role for protein kinase C in urokinase (uPA)-induced smooth muscle cell (SMC) migration, and to examine specific kinase pathways that may act downstream of PKC to influence the response of SMCs to uPA. Background: Vascular SMC migration is an important component of the development of intimal hyperplasia. During tissue remodeling, uPA is one of the key serine proteases involved and stimulates SMC migration in vitro. Methods: Rat arterial SMCs were cultured in vitro. Linear wound and Boyden microchemotaxis assays of migration were performed in the presence of uPA (1-100 nM) with and without general PKC inhibitor, GF109203X (GFX, 1-10 nM), PKC-alpha inhibitor, Goe6976 (1μM) and PKC-delta inhibitor, rottlerin (3μM).
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