Abstract
To use liquid chromatography-mass spectrometry (LC-MS) to identify endogenous differential metabolites in the urine of rats with chronic atrophic gastritis (CAG). Methylnitronitrosoguanidine (MNNG) was used to produce a CAG model in Wistar rats, and HE staining was used to determine the pathological model. LC-MS was used to detect the differential metabolic profiles in rat urine. Diversified analysis was performed by the statistical method. Compared with the control group, the model group had 68 differential metabolites, 25 that were upregulated and 43 that were downregulated. The main metabolic pathways were D-glutamine and D-glutamic acid metabolism, histidine metabolism and purine metabolism. By searching for differential metabolites and metabolic pathways in the urine of CAG rats, this study provides effective experimental data for the pathogenesis and clinical diagnosis of CAG.
Highlights
In the registration of weight changes at 16 weeks, normality test was performed first, and one-way ANOVA was used to conduct statistical test of weight between the blank group and the model group, and P
Permutation is the result of 200 permutation tests, PLD-DA permutation test in positive ion mode is R2 = (0.0,0.91), Q2 = (0.0,0) PLD-DA permutation test in negative ion mode is R2 = (0.0,0.9), Q2 = (0.0,0.39) The results showed that the two groups of samples could be significantly separated
Chen jiaolong [9] by using nuclear magnetic metabonomics technology including observation of the stomach meridian the Chronic atrophic gastritis (CAG) treatment, use of electroacupuncture in the rat stomach meridians beam door and foot three mile, found that serum ghrelin level stomach metabolism of liver kidney brain cortex spectral change, the rat gastric mucosa arrangement and the thickness of the gastric mucosa has different degrees of improvement, ghrelin and substance P expression in serum increased to normal level, serum glucose glycogen content increased, the stomach tissue of glutathione and chemical compound m/z rt (S)-2-Hydroxyglutarate 1-Noradrenaline
Summary
Chronic atrophic gastritis (CAG) is a type of atrophy of gastric mucosal epithelial cells and glands where the number of glands is reduced, the mucosal layer thins, and the mucosal. Urine metabolomics research and innovation teams at Shandong University of Traditional Chinese Medicine in the treatment of major diseases No 220316 and Shandong province key research and development plan 2016CYJS08A01-6“Establishment of Clinical Diagnosis and Treatment Standard System of Precise Prescription for the Prevention and Treatment of Gastric Precancerous Lesions by Jingfang Pinellia Xiexin Decoction
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
