Urine gamma-glutamyl transferase in rat kidney toxicology: Nephropathy by repeated injections of mercuric chloride. Effects of sodium selenite

Abstract

Groups of 5 male and 5 female Cobs CD rats weighing 250–350 g were injected intraperitoneally, daily for 15 days, with 5 μmol HgCl 2/kg, 5 μmol Na 2SeO 3/kg, or (5 μmol HgCl 2 + 5 μmol Na 2SeO 3)/kg in a 10 ml/kg vol. of saline. Control animals were injected with saline only. Injection of saline or sodium selenite produced neither modification of diuresis, nor of urine elimination of sodium, potassium, chloride, phosphates, urea, creatinine and gamma-glutamyl transferase (GGT). Injection of mercuric chloride induced a massive increase of urine GGT, diuresis and phosphaturia and a decrease of kaliuria and natriuria. Those effects reflect a kidney tubular lesion which seems to be more severe in males than in females. Injection of mixed sodium selenite and mercuric chloride or separate injection of both compounds had similar effects. In both sexes, urine GGT elimination was delayed and about 2 times lower than with HgCl 2 alone. In females, the other urine parameters were almost normal whereas in males, diuresis and phosphaturia were slightly increased and kaliuria decreased. The observation of urine GGT elimination attests, in vivo, that sodium selenite decreases tubular toxicity of mercuric chloride and resulting kidney function disturbances.