Abstract
Objective: We compared leukocyte counts obtained by cytometric analysis and Fuchs–Rosenthal (FR) chamber counting in different proportions of lymphocytes (Lym%) suspensions and cerebrospinal fluid (CSF). Design and methods: UF-100 (UF) was evaluated. For preparation of cell suspensions, gradient density centrifugation method was used. Results: The regression equation for UF and FR chamber counting of the cell suspensions was y = 0.88 x + 18.8 WBC/μL ( r = 0.832, n = 106). For a few high Lym% samples, markedly underestimated WBC counts were obtained by UF. Conclusions: Underestimated WBC count is due not to systematic error but to random error. Counts of the “other” population by UF may be useful for detection of underestimated samples.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have