Urinary subclasses of glutathione-s-transferase (α и Ï€) as an indicator of early damages in human kidney tubular cells

Abstract

The aim of this work was to obtained information about the possibility of differentiating (with a- and TE-GST) between cellular damage at different levels of the tubular system in specific forms of acute renal failure (ARF) - ischemic vs. obstruction. The study focused specifically on differencing between acute allograft rejection and cyclosporineA induced nephrotoxicity. PATIENTS AND METHODS. Twenty two patients, hospitalized at the Department of Nephrology, Clinical centre-Skopje, suffering from ARF with the average age 48.1±5.6 years were studied. Forty five recipients with renal allograft were followed daily from the time of transplantation until discharge from hospital and then twice weekly for a total 3 months. Urine sample were obtained from patients with ARF as an ischemic tubular necrosis and acute obstructive renal insufficiency and patients with renal transplantation. The concentration of a- and TE-GST in urine were measured following the method of quantitative EIA (Biotrin Int.LTD). The serum samples from transplanted patient were measured for creatinine and CystatinC. RESULTS. Significant differences of p-GST values between ischemic ARF and acute renal obstruction were observed, while they were insignificant for the values of a-GST. There were no significant differences for a-GST values of all three samples in both groups with ARF. In the cases of acute rejection after transplantation, the release of a-GST into the urine is limited, while that of the TE-GST isoenzyme is extensive. In contrast to the case of acute rejection, in the early stages of CsA induced nephrotoxicity a significant amount of a-GSTs excreted into the urine, but no significant elevation of urinary TE-GST is seen. Scripta Scientifica Medica 2011;43(2): 107-111