Abstract
Urinary steroids of 12 children were assayed as their MO-TMSi-derivatives by gas-liquid chromatography on open tubular columns coated with SE 30 as stationary phase. Extracts were separated on SEPHADEX LH 20, before cleavage of conjugates, into free steroids, glucosiduronates, and sulfates.-The output of 11-desoxy-, and particularly ll-oxygenated-17-oxo-steroids was markedly reduced, most significantly in the glue.-fraction; the decrease in 17-hydroxycorticosteroids was mainly due to low tetrahydrocortisone and cortolones; the percentage contribution of 5-alpha-tetra-hydrocortisol was decreased to 40% of normal. Under ACTH-stimulation the 5-alpha/5-beta ratio for androsterone/etiocholanolone and 5-alpha-tetrahydrocortisol/tetrahydrocortisol was further lowered (.3) than without (.5) or later during thyroid replacement therapy (1.1;.9). The percentage distribution of 17-alpha-hydroxycorticosteroide in fractions gluc-sulf.-free was 70/20/10%, under ACTH 60/20/20%.- These results comply with recent observations in adults indicating a diminished activity of 11-beta-hydroxy-steroid dehydrogenase, 5-alpha-reductase, and glucuronyl-transferase-system in hypothyroidism. Supported by Deutsche Forschungsgemeinschaft Bl 95/4.
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