Abstract
Excretion of urinary N-acetyl beta-D-glucosaminidase (NAG) and its isoenzyme patterns were studied in two groups of patients with rheumatoid arthritis (RA) and in normal control subjects. Urine samples were collected from 30 seropositive RA patients, 19 seronegative RA patients, and 15 normal healthy subjects. All the patients and normal subjects were assessed to have normal liver and kidney functions. A small portion of the urine sample was dialyzed against 0.01 M phosphate buffer, pH 7.0 and NAG activity was monitored. Mean +/- SD values of urinary NAG in seropositive RA patients, in seronegative RA patients and in normal healthy subjects were found to be 4.20 +/- 3.73 U/g creatinine, 2.96 +/- 2.11 U/gm creatinine, and 1.71 +/- 0.6 U/g creatinine, respectively. The mean urinary, NAG value in RA patients was found to be significantly higher (P < 0.05) in seropositive RA compared to the mean NAG value in normal healthy subjects and patients with seronegative RA when analyzed by one way ANOVA and Tukey-HSD test. The mean proportion of isoenzyme form B to isoenzyme form A in seropositive RA patients was also found to be significantly different (P < 0.05) from the mean proportion of these forms in normal healthy subjects and seronegative RA patients. There also appears to be a correlation between the concentration of urinary NAG and severity of the disease in seropositive RA.
Highlights
N-Acetyl β-D-glucosominidase (NAG) is a lysosomal enzyme, which is mainly present in proximal convoluted tubules
Tukey-HSD test at 5% significance level further revealed that the mean NAG value in the seropositive rheumatoid arthritis (RA) group was significantly different from the mean NAG values in normal control group and seronegative RA group
This study shows that total activity of NAG in urine is significantly elevated in seropositive RA patients compared to normal healthy subjects
Summary
N-Acetyl β-D-glucosominidase (NAG) is a lysosomal enzyme, which is mainly present in proximal convoluted tubules. It has been reported that urinary NAG levels are elevated in adjuvant arthritis (Franch et al, 1990) and there appears to be a positive correlation between urinary NAG excretion and severity of the disease. The present study was undertaken to investigate the excretion of urinary NAG and the pattern of its isoenzymes in different types of rheumatoid arthritis (RA) and compare these values with those in normal healthy subjects. Another objective was to see if there was any correlation between urinary levels of NAG and severity of the disease
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