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Abstract
BackgroundIgA nephropathy (IgAN) is the most common primary glomerulonephritis worldwide. Urinary micro-RNA (miRNA) level is increasingly reported to as non-invasive markers of various kidney diseases. We aim to identify urinary miRNA targets for the diagnosis of IgAN.MethodsIn the development cohort, we performed complete miRNA profiling of urinary sediment in 22 patients with IgAN and 11 healthy controls (CTL). Potential miRNA targets were quantified by a separate validation cohort of 33 IgAN patients and 9 healthy controls.ResultsIn the development cohort, we identified 39 miRNA targets that have significantly different expression between IgAN and CTL (14 up-regulated, and 25 down-regulated). Among the 8 miRNA targets chosen for validation study, urinary miR-204, miR-431 and miR-555 remained significantly reduced, and urinary miR-150 level was significantly increased in the IgAN as compared to CTL. The area-under-curve of the receiver operating characteristic (ROC) curve for urinary mi-204 level for the diagnosis of IgAN was 0.976, and the diagnostic performance of combining additional miRNA targets was not further improved. At the cut-off 1.70 unit, the sensitivity and specificity of urinary miR-204 was 100 and 55.5%, respectively, for diagnosing IgAN.ConclusionsUrinary miR-150, miR-204, miR-431 and miR-555 levels are significantly different between IgAN and healthy controls; urinary miR-204 level alone has the best diagnostic accuracy.
Highlights
IgA nephropathy (IgAN) is the most common primary glomerulonephritis worldwide
Our objective is to identify a panel of urinary miRNA targets that could be used for the diagnosis and risk stratification of IgA nephropathy
Among the miRNA targets that are highly expressed in the urinary sediment, we identified 39 miRNA targets that have significantly different expression between IgAN and CTL groups (14 up-regulated, and 25 downregulated) (Table 3)
Summary
IgA nephropathy (IgAN) is the most common primary glomerulonephritis worldwide. Urinary microRNA (miRNA) level is increasingly reported to as non-invasive markers of various kidney diseases. We aim to identify urinary miRNA targets for the diagnosis of IgAN. Immunoglobulin A nephropathy (IgAN) is the most common primary glomerulonephritis in the world [1]. The clinical course of IgAN, is highly variable. The major clinical tools for predicting prognosis of IgAN are baseline renal function, proteinuria, and histological grading [2,3,4]. Non-invasive biomarkers are much needed for risk stratification as well as monitoring of response to treatment. MicroRNA (miRNA) is a group of short noncoding RNA molecules with a length around 22 bases that
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