Abstract
Esophageal cancer (EC) including esophageal squamous cell carcinoma (ESCC) and adenocarcinoma (EAC) generally exhibits poor prognosis; hence, a noninvasive biomarker enabling early detection is necessary. Age- and sex-matched 150 healthy controls (HCs) and 43 patients with ESCC were randomly divided into two groups: 9 individuals in the discovery cohort for microarray analysis and 184 individuals in the training/test cohort with cross-validation for qRT-PCR analysis. Using 152 urine samples (144 HCs and 8 EACs), we validated the urinary miRNA biomarkers for EAC diagnosis. Among eight miRNAs selected in the discovery cohort, urinary levels of five miRNAs (miR-1273f, miR-619-5p, miR-150-3p, miR-4327, and miR-3135b) were significantly higher in the ESCC group than in the HC group, in the training/test cohort. Consistently, these five urinary miRNAs were significantly different between HC and ESCC in both training and test sets. Especially, urinary miR-1273f and miR-619-5p showed excellent values of area under the curve (AUC) ≥ 0.80 for diagnosing stage I ESCC. Similarly, the EAC group had significantly higher urinary levels of these five miRNAs than the HC group, with AUC values of approximately 0.80. The present study established novel urinary miRNA biomarkers that can early detect ESCC and EAC.
Highlights
Early detect gastric cancer[19]
Stage I and T1 stage were found in 37.2% and 48.8% of esophageal squamous cell carcinoma (ESCC), respectively
Given that circulating miRNAs in body fluids are protected from degradation by binding to RNA-binding proteins such as lipoproteins or being contained in extracellular vesicles such as microvesicles and exosomes[24,25,26], these miRNAs are potential candidates for minimally invasive biomarkers for cancer diagnosis[27,28]
Summary
We aimed to establish novel urinary miRNA biomarkers for the early detection of EC including ESCC and EAC
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