Urinary Kallikrein and Urinary Prostaglandin E2 in Genetically Hypertensive Mice

Abstract

SummaryWe have evaluated urinary kallikrein activity and urinary prostaglandin E2 (PGE2) immunoreactivity in Schlager high blood pressure (HBP) and low blood pressure (LBP) mice. Urinary kallikrein activity was measured by a colorimetric method employing α-N-p-tosyl-L-arginine methyl ester HCl (TAME) as substrate. It was shown that urinary TAME esterase activity in HBP and LBP mice was probably due to a single enzyme and that this enzyme appeared to be urinary kallikrein. Urinary PGE2 immunoreactivity was determined by a specific radioimmunoassay. Urinary kallikrein activity was significantly elevated and urinary PGE2 immunoreactivity was significantly reduced in HBP compared to LBP mice. High urinary kallikrein activity in HBP mice resembles situations such as human primary aldosteronism, where hypertension is produced by mineralocorticoid excess. Low urinary PGE2 levels in HBP mice, however, compares to the situation observed for human essential hypertension. The HBP mouse thus has alterations in physio...