Urinary immunoreactive estrogen and pregnanediol-3-glucuronide during the normal menstrual cycle of the female lowland gorilla (Gorilla gorilla).

Abstract

Urine samples were taken daily during 22 menstrual cycles of six normal adult female gorillas. Urine was analyzed for total immunoreactive estrogens (Et) and pregnanediol-3-glucuronide (PdG) and indexed by creatinine (Cr). An average cycle length of 32 ± 1 days (mean ± SE) with a range of 25-42 days is reported. Estrogen values range from 4 to 128 ng/mg Cr and show a midcycle peak and a midluteal rise. PdG values range from 0.01 to 2.4 μg/mg Cr and display a low, flat follicular phase followed by a luteal elevation. The follicular phase is 19.5 ± 1 days in length (range 11-30 days) and accounts for the variation in cycle length. The luteal phase is 12.3 ± 0.3 days long (range 10-14 days). In contrast to previous studies, PdG levels rise two days before the estrogen peak. The results from the present study are compared with information available on the gorilla, chimpanzee, and human. The accuracy of various alignment methods is discussed, as well as the importance of the methods presented in this study for the captive propagation of gorillas.