Abstract
The urinary acidic glycosaminoglycans (AGAG) of Werner's syndrome were isolated, purified and characterized by gel-chromatography, cellulose acetate electrophoresis, chemical analysis, streptomyces hyaluronidase susceptibility and viscometry. The AGAG appeared at the first peaks of the 0.6 M and 0.8 M fractions obtained through Sephadex G-100 were mainly a hyaluronic acid (HA). HA was composed of 16% of the urinary AGAG. The AGAG at the first peak had the maximum molecular weight of 360 000 in the 0.8 M fraction followed by lesser molecular weights in the other fractions.
Published Version
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