Abstract
Some time ago in a study in this laboratory1 dealing with the influence of ether anesthesia on the acid-base balance in dogs it was noted that when silver nitrate was added to urine collected following the anesthesia for the chloride estimation, the silver was reduced and a black precipitate formed before the acid indicator was added. This observation led us to investigate the influence of the ether anesthesia on the excretion of ascorbic acid as determined by titration with 2:6-dichlorophenolindophenol.Female dogs were employed. The urine was collected by catheterization into a bottle containing a few drops of glacial acetic acid, after which sufficient 20% trichloracetic acid was added to give a 5% trichloracetic acid concentration. This was then titrated into a known amount of 2:6-dichlorophenolindophenol. The dye was standardized with crystalline ascorbic acid (Merck) according to the procedure of Birch, Harris and Ray.2 It was found, however, that when urine was allowed to stand even for short period...
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