Abstract
The purpose of this study was to investigate the effects of parenteral uridine administration on acetylcholine release by in vivo microdialysis in striatum of freely‐moving rats.Microdialysis probes inserted into left striatum of rats were perfused with artificial cerebrospinal fluid. After collection of 3 basal microdialysate samples, uridine was injected intraperitoneally. In additional experiments, P2Y receptor antagonism was achieved by intracerebroventricular injection of suramine 15 min prior to uridine administration. Levels of uridine nucleotides, choline and acetylcholine in microdialysate samples were analyzed by High Performance Liquid Chromatography.Intraperitoneal administration of uridine enhanced brain extracellular uridine diphosphate (UDP), uridine triphosphate (UTP) levels and acetylcholine levels, while extracellular choline levels decreased after uridine. Suramine, a non‐selective P2Y receptor antagonist, blocked the alterations in extracellular acetylcholine and choline levels.Our data provide the first in vivo evidence for the existence of pyrimidinergic transmission in the brain which can be enhanced by exogenous uridine administration. We also show for the first time that increased pyrimidinergic transmission enhances cholinergic neurotransmission, possibly by activating P2Y receptors.This work was supported by grants from TUBITAK 108S354.
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