URG11 Regulates Prostate Cancer Cell Proliferation, Migration, and Invasion.

Bin Pan,Jianli Zhen,Haiping Liu,Lijun Qu,Weifeng Zhong,Youke Wu,Yutong Li,Chuanrong Zeng,Hongmei Zhou,Yunlin Ye

doi:10.1155/2018/4060728

Abstract

Upregulated gene 11 (URG11), a new gene upregulated by hepatitis B virus X protein, is involved in the development and progression of several tumors, including liver, stomach, lung, and colon cancers. However, the role of URG11 in prostate cancer remains yet to be elucidated. By determined expression in human prostate cancer tissues, URG11 was found significantly upregulated and positively correlated with the severity of prostate cancer, compared with that in benign prostatic hyperplasia tissues. Further, the mRNA and protein levels of URG11 were significantly upregulated in human prostate cancer cell lines (DU145, PC3, and LNCaP), compared with human prostate epithelial cell line (RWPE-1). Moreover, by the application of siRNA against URG11, the proliferation, migration, and invasion of prostate cancer cells were markedly inhibited. Genetic knockdown of URG11 also induced cell cycle arrest at G1/S phase, induced apoptosis, and decreased the expression level of β-catenin in prostate cancer cells. Overexpression of URG11 promoted the expression of β-catenin, the growth, the migration, and invasion ability of prostate cancer cells. Taken together, this study reveals that URG11 is critical for the proliferation, migration, and invasion in prostate cancer cells, providing the evidence of URG11 to be a novel potential therapeutic target of prostate cancer.

Highlights

Prostate cancer (PCa) is one of the most common cancers of urinary system and the second most common cause of cancer-related death in men in western world [1]
Informed consent was received from all participated subjects prior to the study. 68 cases of human tissue samples of prostate cancer and 74 cases of benign prostatic hyperplasia were obtained from prostate patients in the First Affiliated Hospital of Jinan University and Sun Yatsen University Cancer Center, from Jun 2010 to Jun 2013
Positive expression ratio in prostate cancer group (PCa) was 70.6% (48/68) and that in benign prostatic hyperplasia (BPH) group was 21.6% (16/74), suggesting that Upregulated gene 11 (URG11) levels were significantly upregulated in prostate cancer tissues (p < 0.01)

Summary

Introduction

Prostate cancer (PCa) is one of the most common cancers of urinary system and the second most common cause of cancer-related death in men in western world [1]. Metastasis is the leading cause of morbidity and mortality of prostate cancer. Cytokine induced cancer cell specific oriented homing and increased adhesion opacity to organic microvascular endothelium, tumor angiogenesis and lymphangiogenesis, and other factors combine together to impact the process [4]. Among these factors, epithelialmesenchymal transition (EMT) is an important event for cancer cell movement, initiating the initial phase of metastasis [5]

Objectives

Methods

Results

Conclusion