Abstract
Polyacrylamide-allyl glycidyl ether [poly(AAm-AGE)] cryogel monolith was prepared for covalent immobilization of urease enzyme and aimed for usage of urea depletion from human serum by immobilized urease. Immobilization was succeeded via epoxy groups of allyl glycidyl ether with an immobilization yield of 71.9 ± 2.65%. Characterization was performed by Fourier Transform Infrared Spectroscopy (FTIR), Scanning Electron Microscopy (SEM), Energy Dispersive X-Ray (EDX) analysis and swelling analysis to reveal functionalities and morphological, elemental and macroporous structure of the cryogel. Effects of pH, temperature and urea concentration on catalytic activity profiles of free and immobilized urease were investigated. Thermal and storage stabilities of free urease were enhanced upon immobilization. Additionally, urea removal from artificial human serum was achieved by 92% and immobilized urease was reusable up to ten reuses.
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