Urease Enzyme Biosensor for Water Pollution Detection

Abstract

Urea is the most containing nitrogen substance in the Urine of mammals. It plays an important role in the metabolism of nitrogen containing compounds by animals and human. A Biosensor for Urea was made by immobilizing Urease enzyme and Bromo stain which both obtained from sigma- Aldrich company on the microparticales surface were prepared through encapsulation successively by LBL self- assembly process of polyelectrolyte used PASS & PAH Polymers of eight layers. The size of nanoparticales was (5.05 – 5.87 nm). In other case some of these microparticales were removed there cores by HCL acid and filled with Bromo stain which is sensitive to change in PH. The microparticales were prepared and determined there size, layers under the SEM microscopy. The temperature, PH buffer concentration, Urease enzyme concentration, and enzyme immobilizing procedure were investigated. Microparticales prepared with Urease enzyme gives high sensitive response time 1-2 min. This Biosensor can use to determine the pollution in water and these microparticales made possible Biosensor to carry inside many medical materials in the future.