Abstract
Abstract Urease activity of the 26 soil samples, collected from semiarid regions of northwestern India ranged from 6.5 to 103.6 μg urea‐N hydrolyzed g‐1 soil h‐1 and was related to organic carbon and clay contents of the soil. Soil urease activity in different soils was represented by Michaelis‐Menten‐type kinetics. Hydrolysis of urea over time followed first‐order kinetics in the three representative soils. The first‐order rate constant k increased with rise in temperature from 8 to 35°C and was greater at field capacity moisture regimes than at the moisture regime on either side of field capacity. Among the three soils, rate of urea hydrolysis was fastest in the soil with the highest urease activity and organic carbon. Thermodynamic properties (energy of activation, free energy, entropy, enthalpy) have also been calculated for the selected soils. Of the two urease inhibitors, N‐(n‐butyl)thiophosphoric triamide (NBPT) was more effective in inhibiting urea hydrolysis than phenyl phosphorodiamidate (PPD).
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