Abstract
In cement-based cementitious materials, high-porosity areas tend to expand microcracks. To counter this problem, microbially induced calcite precipitation (MICP) was suggested to fill the pores. However, it is unclear how to control the supply of urea to increase the MICP efficiency of Bacillus sp. Therefore, in this study, the microbial growth rate, urease activity, and urea-to-carbonate conversion efficiency were determined under different urea concentrations. When the urea concentration was 0.15 mol/L, the growth rate was 0.22 h−1, the cell number reached 4 × 109 colony-forming units (CFUs) mL−1, and the urease activity was approximately 0.12 mg g-1h−1. Additionally, the conversion efficiency reached 40%, following the rule of 0.51 + 0.08 e0.5x. The electron-transfer rate of MICP measured via cyclic voltammetry was 6.6 × 10-6 cm s−1. It is based on nutrients, Bacillus sp., and urea and aims to develop a self-healing coating by reducing the porosity and improving its apparent impermeability.
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