Abstract
The staphylococcal α-hemolysin (αHL) protein nanopore is under investigation as a fast, cheap detector for nucleic acid analysis and sequencing. Although the discrimination of all four bases of DNA by the αHL pore has been demonstrated, the analysis of single-stranded DNAs and RNAs containing secondary structure mediated by base pairing is prevented because these nucleic acids cannot be translocated through the pore. Here, we show that a structured 95-nucleotide single-stranded DNA and its RNA equivalent are translocated through the αHL pore in the presence of 4 M urea, a concentration that denatures the secondary structure of the polynucleotides.
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