Abstract
Fluore sce nt Pseudomonas species produce pyove rdine -type siderophores with a high pote ntial to dissolve ; bind and transport uranium in the e nvironment. Ide ntification of Pseudomonas fluorescens SHA 281 was confirme d by 16S rDNA sequ e ncing. The pyoverdine produce d by the tested strain was e xtracte d and purifie d using XAD-4 Ambe rlite re sin and its chemical structure was confirme d using NMR and CA-MS analyses. The ge l e lectrophoresis of pyoverdine appeare d low molecular we ight with one prote in band at ~12.5 kDa. The ge ne responsible for pyoverdine production was ide ntifie d using PCR technique . Unde r optimum conditions for comple xation, it was found that pyoverdine comple xe d with about 90.48 % and 86.41 % of U (VI) from monazite and (Th-U) concen trate, respectively.
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