Abstract
Bioremediation of uranium (VI)-contaminated aquifers was investigated by using bacteria isolated from a uranium tailing in South China. The bacteria were identified as Acinetobacter on the basis of physiological, biochemical tests and 16S rDNA sequence homology analysis. The protein concentration in the supernatant declined from 50.26 μ g/mL to 40.01 μ g/mL after uranium removal, and the activity of bacterial phosphatase increased from 36.04 U/L to 39.81 U/L. The FTIR, SEM-EDS and XRD demonstrated the involvement of functional groups in U (VI) binding, U accumulated on the surface of the bacteria and the precipitated is Uranyl Sulfite Hydrate. The results from this study demonstrate the potential of the U tolerant, bacterium Acinetobacter sp. USC B2 isolate might be considered as promising candidates in the design of uranium bioremediation strategies.
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