Uranium sequestration abilities of Bacillus bacterium isolated from an alkaline mining region

Abstract

The present study elaborates uranium sequestration by bacteria from alkaline wastewaters. In the investigation, a few bacterial strains were isolated from alkaline uranium mine water and were tested for uranium sequestration properties 16S rRNA analysis assigned the 10 bacterial isolates to 4 genera of Actinobacteria and Firmicutes. Among all the isolates tested, the strain Bacillus aryabhattai (TP03) has shown superior sequestration capacity at 5 and 10 mg/L U in 1 mM carbonate-bicarbonate buffer at pH 9.2. At low uranium concentrations (5 mg/L as uranyl carbonate), the strain could sequester ~70% of the uranium in 6 h with a loading capacity of 4.3 mg U/g dry bacterial biomass. Increase in carbonate-bicarbonate buffer concentrations and pH reduced the sequestration capacity. Scanning electron microscopy and energy dispersive X-ray fluorescence spectroscopy studies indicated the presence of uranium with the bacterial biomass. Fourier transform infra-red spectroscopy results confirmed the uranium sequestration by cell membrane phosphate, amide, and carboxyl functional groups. Transmission electron microscopy study showed uranium presence within the cell cytoplasm, thus supporting the hypothesis on active metabolism-dependent bioaccumulation of uranium. The kinetics study of uranium sequestration was well fitted to the pseudo-second-order model. Overall, this study infers that the isolated alkaliphilic bacteria from the mine waters have significant sequestration property for treating uranium-containing alkaline wastewaters.