Abstract
The objective of this study was to determine the influence of coating of radiolabeled poly(methylmethacrylate) nanoparticles (PMMA nanoparticles) with surfactants on their uptake by bovine microvessel endothelial cell cultures (BMEC cultures). For this purpose, BMEC cultures were grown in 24-well culture plates, where they formed confluent monolayers 10–12 days after seeding. The nanoparticle suspensions were then incubated with the cell cultures at 37°C, and the radioactivity within the cell cultures and the supernatant was measured after 30 min, 2 h and 6 h. The rates of uptake of the coated nanoparticles and of a control group of uncoated nanoparticles were compared.
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