Abstract
Cell suspension cultures of soybean were synchronized by accumulation of cells at the G1/S boundary with 5-fluorodeoxyuridine. Upon removal of fluorodeoxyuridine and addition of thymidine, viable cells resume growth and enter mitosis with increased synchrony. Protoplasts were isolated from cells at varying times following release from inhibition and assayed for uptake of 3H-pBR322 plasmid DNA. Uptake into DNase-resistant, trichloroacetic acid —insoluble form was 2.5 to 4.5-fold higher in S phase than in G1 or G2 phase protoplasts. Endogenous soybean deoxyribonuclease activities did not vary over the cell cycle.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
