Abstract
Abstract The existence of transport of butyl-β-carboline-3-carboxylate (βCCB) into rat cerebral cortex was examined in vitro . Accumulation of βCCB was observed within fragments of rat cerebral cortex at 37°C, reaching levels of 9200 pmol βCCB/mg of protein in 30 min. In crude synaptosomal fraction the uptake was rapid, reaching equilibration after 2 min. Kinetic analyses demonstrated that the mechanism was saturable with an estimated K M of 30 μM and a maximum influx of 2680 pmol/mg of protein/min. The transported material was accumulated inside the synaptosomal vesicles and was identified as βCCB by HPLC. Under our experimental conditions the βCCB uptake was not Na + -dependent. The cellular uptake of βCCB in brain tissue supports the hypothesis that this molecule may play a functional role in the brain.
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