Abstract
The seawater chemistry of potentially toxic metals can affect their availability to marine organisms. Investigation of the relationship between metal chemistry and metal bioavailability has progressed slowly due to difficulties in controlling and measuring metal speciation in uptake media. Recent work with strong metal chelators such as NTA and EDTA has allowed a closer examination of how metal chemistry relates to biological accumulation and toxicity. 1–3 However, the presence of a strong chelator at membrane transport sites and the possible alteration of microenvironments by strong chelators could create unnatural uptake behavior. This study presents another method for stabilizing metal chemistry in accumulation experiments. A cation exchange resin was used to study Mn 54 accumulation by a small bivalve Donax variabilus. The resin proved an effective method for buffering manganese chemistry in seawater and could provide a useful tool to look for subtle effects present in other metal buffered seawater systems.
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