Abstract
During the shift from exponential to stationary phase, areas of the yeast vacuolar membrane or tonoplast become depleted of intramembranous particles. Lipid bodies enter the vacuole through invaginations of these particle-poor regions. During uptake, the lipid bodies are enclosed in a membrane derived from the particle-depleted region of the tonoplast. The vesicles are sealed and released into the vacuole when the tonoplast fuses with itself behind the lipid body.
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