Abstract

Wheat root tips from germinatyed seedlings were incubated in labeled D-glucose-U- L- 14C for periods up to 30 min. Uptake of activity into glucose-1-phosphate, glucose-6-phosphate, sucrose and UDP-glucose was monitored. Extremely low, but significant, activities were found in ADP-glucose and GDP-glucose. A Golgi fraction was isolated. Activity was found in a particulate form but it could be solubilized in mild base or acid. Sephadex chromatography of the base solubilized material demonstrated that it had high mol. wt. Acid hydrolysis yielded mainly radioactive xylose, arabinose, glucose, galactose, with lesser quantities of mannose and uronic acids.

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