Uptake of estradiol by the rat pineal organ. Effects of cervical sympathectomy, stage of the estrous cycle and estradiol treatment

Abstract

Rat pineal organs of spayed rats took up and retained estradiol in vitro up to 32-fold the concentration present in the incubation media. This phenomenon was maximum at 37°C and after 2-h incubations. Most (86–91%) of [ 3H] radioactivity recovered from the incubated pineals was identified as estradiol by thin-layer chromatography. Treatment with dextran-coated charcoal of nuclei-free pineal homogenates incubated with [ 3H] estradiol of different SA uncovered a high affinity, low capacity binding of estradiol to cytosol components. Uptake of estradiol by the nuclear fraction also proceeded in a saturable fashion. Similar findings were made in uterine homogenates of spayed rats. Estradiol uptake by the pineal organ and the uterus of cycling rats varied as a function of the stage of the estrous cycle, maxima being observed in diestrus and minima in proestrus. The administration of a priming dose of estradiol benzoate to spayed rats caused high affinity binding components of the pineal cytosol to increase by about 150%. Nuclear binding of estradiol was also increased by the estradiol priming dose. Pineal denervation, i.e., by superior cervical ganglionectomy, caused pineal estradiol uptake to decrease significantly by about 20%. These data suggest that the early steps of estradiol action on the pineal organ may resemble those of the uterus.