Abstract
Rat brain synaptosomes incubated in modified Krebs-Ringer media accumulated 45Ca in amounts dependent upon the medium calcium concentration. At 37 °C, 45Ca uptake was increased by 19% upon the application of electrical pulses. Uptake was effectively inhibited by ruthenium red, uncouplers of oxidative phosphorylation, antimycin A and rotenone, and fluorocitrate while oligomycin and ouabain increased 45Ca uptake. Transfer to a medium low in Na + led to marked increase in 45Ca uptake and this effect was also found in the presence of rotenone and arsenate. The results indicate the requirements of metabolic energy for a component of calcium uptake by synaptosomes. Effects of low Na + incubation and electrical stimulation may be due to events at the outer synaptosomal membrane though the possibility of direct effects at the mitochondrion have not been excluded.
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