Uptake, Elimination, and Biotransformation of 17α-Ethinylestradiol by the Freshwater Alga Desmodesmus subspicatus

Abstract

Bioconcentration and transformation of the potent and persistent xeno-estrogen 17α-ethinylestradiol (EE2) by organisms at the basis of the food web have received only little research attention. In this study, uptake, elimination, and biotransformation of radiolabeled EE2 ((14)C-EE2) by the freshwater green alga Desmodesmus subspicatus were investigated. The alga highly incorporated radioactivity following (14)C-EE2 exposure. Up to 68% of the test compound was removed from the medium by D. subspicatus within a rather short time period (72 h C(algae)/C(water): 2200 L/kg wet weight). When the algae were transported to clear medium, a two-stage release pattern was observed with an initially quick elimination phase following slower clearance afterward. Interestingly, D. subspicatus brominated EE2 when bromide was available in the medium, a transformation process demonstrated to occur abiotically but not by algae. The consequence of the presence of more hydrophobic mono- and dibrominated EE2 in the environment remains to be further investigated, as these products were shown to have a lower estrogenic potency but are expected to have a higher bioaccumulation potential and to be more toxic than the mother compound.