Abstract
Summary Expiants of tobacco pith (Nicotiana tabacum L.) grown on Murashige and Skoog's medium labelled with 14C-indoleacetic acid accumulate similar small amounts of auxin regardless of external concentration. The labelled products produced are similar chromatographically to those reported in the literature for whole plants and plant parts. Isolated tobacco pith gradually loses its tendency to transport auxin basipetally during culture despite subsequent appearance of morphological polarity; it is concluded that the auxin gradients responsible for polarity are imprinted soon after transfer to the growth medium.
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